Month: August 2025

In a basement from the Biomedical Research Park in Barcelona, ​​between liquid nitrogen clouds, an incalculable value treasure is saved: an ark of Noah of the 21st century. It does not contain couples of animals, but thousands of small tubes at -196 ° C that retain life. It is the Cryozooa pioneer biobanco that stores cell lines of hundreds of species, many of them to the edge of extinction. It is not an achievement, but a warning. At the head of this initiative is the renowned molecular biologist Tomàs Marquès-Bonet, one of the Greater world experts in genomics of great worlds. As the world has collected, This project is not a great achievementbut a last use resource in the event that the main species of our planet are extinguished. This is explained by the researcher himself: Recovering species with these techniques is the failure of society, but it is amazing to be able to do it. The first must be to preserve in your habitat the animals that remain alive. And when everything else has failed, it is better to have these banks than not to have them, like an ace in the manga Of a biopsy to cell immortality. The concept, inspired by the famous San Diego Frozen Zoo, is as elegant as powerful. The Cryozoo team collaborates with about twenty European zoos and aquariums to obtain small tissue samples, often during routine veterinary reviews. In this way, with a millimeter of leather you can create a stock of cell lines and keep them forever. The process is surprisingly pragmatic. Zoos send biopsies in tubes with a conservation medium. A complex cold chain is not always needed; Sometimes, as in the case of a stranded whale in Valencia, a little serum is enough to start. In the laboratory conservation is consumed. Once the fabric reaches the laboratory, Technicians cultivate cellsallowing them to divide and multiply to form a homogeneous population that is called ‘cell line’. Reprogramming to stem cells. The most revolutionary step is reprogramming. They can take a skin cell and, by laboratory techniques, return it to a pluripotent state, turning it into a stem cell of induced pluripotentiality (IPSC). “A stem cell is a pluripotent cell, which means that it can become what you want,” says Marquès-Bonet. And once this is achieved, the last step of cryopreservation of both cell lines and IPSC in liquid nitrogen is reached, where they can remain viable for decades, waiting for the science of the future to need them. A technique similar to that used for human embryo conservationfor example, in fertility processes. Currently, Cryozoo already houses more than 2,000 samples of almost 300 species, which have generated 350 high quality cell lines. Among its “treasures” are Montseny Triton cells (the most threatened amphibian in Europe), the Pyrenean frog, the ORYX DAMMAH (A species already extinct in nature) and even the rhinoceros Pedro, the longest in Europe, deceased in 2023. Quality on quantity. What distinguishes Cryozoo from other initiatives is not its size, but its obsession with quality. And it is that the bank’s goal is not to have the more cell lines the better, but to have the best and most viable. To achieve this, they have implemented a step that they consider crucial and that makes them unique: sequence the complete genome of each cell line they create. In this way, they ensure that the genome of the cultivated cell is a faithful representation to the original animal without genetic aberrations that have occurred in the laboratory. AND the fact of sequencing it It is also a great advance for science, because on many occasions it is the first time that this technique is done in a specific species. Something that will be in a repository that any researcher can consult. They want to avoid using these cells. With the ability to convert skin cells into ovules and sperm, the question is inevitable: is the ultimate goal of ‘de -sextinction’? But researchers have it clear: it is a red line that they never want to pass. Although technology has already allowed to bring functionally extinct species such as the Huron of black legs or the Przewalski horse, the Cryozoo team considers that its function is to be custodians of the genetic material, not to execute reproduction. They would only make their cells available to a project of this caliber if it had the validation of the International Union for Nature Conservation (IUCN) and a global consensus. Cloning is not the step. Although it can be attractive to make ‘photocopies’ of animals in a laboratory, the reality is that today It is a expensive and inefficient process. The real effort of the researchers today lies in preserving ecosystems so that animals live in them and reproduce naturally. Without man having to intervene. A cell bank to save animals … and also humans. The value of Cryozoo does not only reside in that distant possibility of resuscitating species. Its applications are immediate and revolutionary for current research. And it is that diseases can be studied without damaging any living being by infecting cells with a pathogen to see how cells react. But it goes further, being able to create ‘mini organs’ to investigate the biology of some species, test drugs safely or investigate human diseases in the genetics of these animals. A hope for an uncertain future. The changes that succumb to our planet can cause in the future to be a real climatic emergency. That is why we prepare the ‘end of the end of the world‘To collect all the seeds of the world, and now we also collect all animals. A genetic library that, in the best stage, we will only consult for pure scientific curiosity and never for a planetary emergency. Images | Gary Bendig Julia Koblitz In Xataka | Apocalypse diet: science already knows what survivors will eat a nuclear war